📦 BioStrings
Init
DNA
# DNA
x <- DNAString("actttGtag")
is(x)
## [1] "DNAString" "XString" "XRaw" "XVector" "Vector" "Annotated"
#转录
x %>% complement() %>% RNAString() -> rna
# 反向互补
x.r <- reverseComplement(x)
x.r
## 9-letter "DNAString" instance
## seq: CTACAAAGT
d <- DNAString("TTGAAAA-CTC-N")
d
## 13-letter "DNAString" instance
## seq: TTGAAAA-CTC-N
#subset
subseq(x, start =1, end = 5)
## 5-letter "DNAString" instance
## seq: ACTTT
#碱基置换
chartr(old = "A", new = "C", x)
## 9-letter "DNAString" instance
## seq: CCTTTGTCG
#DNA_BASES
DNA_BASES
## [1] "A" "C" "G" "T"
#IUPAC_CODE_MAP
IUPAC_CODE_MAP
## A C G T M R W S Y K
## "A" "C" "G" "T" "AC" "AG" "AT" "CG" "CT" "GT"
## V H D B N
## "ACG" "ACT" "AGT" "CGT" "ACGT"
RNA
x <- RNAString("acuuuGuag")
is(x)
## [1] "RNAString" "XString" "XRaw" "XVector" "Vector" "Annotated"
# 逆转录
x %>% complement() %>% DNAString()
## 9-letter "DNAString" instance
## seq: TGAAACATC
# 翻译
codons(x)
## Views on a 9-letter RNAString subject
## subject: ACUUUGUAG
## views:
## start end width
## [1] 1 3 3 [ACU]
## [2] 4 6 3 [UUG]
## [3] 7 9 3 [UAG]
translate(x)
## 3-letter "AAString" instance
## seq: TL*
#dna -> rna:T <-> U
DNAString("actttGtag") %>% RNAString()
## 9-letter "RNAString" instance
## seq: ACUUUGUAG
#rna -> dna:U <-> T
rna %>% DNAString()
## 9-letter "DNAString" instance
## seq: TGAAACATC
AA: Amino acid
x <- AAString("actttGtag")
is(x)
## [1] "AAString" "XString" "XRaw" "XVector" "Vector" "Annotated"
reads
# all kmer
reads <- mkAllStrings(c("A", "C", "G", "T"), 6)
is(reads)
## [1] "character" "vector" "data.frameRowLabels"
## [4] "SuperClassMethod" "characterORconnection" "character_OR_NULL"
## [7] "atomic" "vector_OR_factor"
all6mer <- DNAStringSet(reads)
all6mer[1]
## A DNAStringSet instance of length 1
## width seq
## [1] 6 AAAAAA
all6mer[[1]]
## 6-letter "DNAString" instance
## seq: AAAAAA
frequence
x <- DNAString("actttGtag")
x.freq <- alphabetFrequency(x)
is(x.freq)
## [1] "integer" "numeric" "vector"
## [4] "data.frameRowLabels" "integer_OR_Linteger" "atomic"
## [7] "vector_OR_factor"
x.freq
## A C G T M R W S Y K V H D B N - + .
## 2 1 2 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0
x.freq <- alphabetFrequency(x, baseOnly = T)
x.freq
## A C G T other
## 2 1 2 4 0
# any 2 kmer freq
dinucleotideFrequency(x)
## AA AC AG AT CA CC CG CT GA GC GG GT TA TC TG TT
## 0 1 1 0 0 0 0 1 0 0 0 1 1 0 1 2
# any 3 kmer freq
trinucleotideFrequency(x)
## AAA AAC AAG AAT ACA ACC ACG ACT AGA AGC AGG AGT ATA ATC ATG ATT CAA CAC
## 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
## CAG CAT CCA CCC CCG CCT CGA CGC CGG CGT CTA CTC CTG CTT GAA GAC GAG GAT
## 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
## GCA GCC GCG GCT GGA GGC GGG GGT GTA GTC GTG GTT TAA TAC TAG TAT TCA TCC
## 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0
## TCG TCT TGA TGC TGG TGT TTA TTC TTG TTT
## 0 0 0 0 0 1 0 0 1 1
# any 4 kmer freq
oligonucleotideFrequency(x, 4)
## AAAA AAAC AAAG AAAT AACA AACC AACG AACT AAGA AAGC AAGG AAGT AATA AATC AATG
## 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## AATT ACAA ACAC ACAG ACAT ACCA ACCC ACCG ACCT ACGA ACGC ACGG ACGT ACTA ACTC
## 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## ACTG ACTT AGAA AGAC AGAG AGAT AGCA AGCC AGCG AGCT AGGA AGGC AGGG AGGT AGTA
## 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
## AGTC AGTG AGTT ATAA ATAC ATAG ATAT ATCA ATCC ATCG ATCT ATGA ATGC ATGG ATGT
## 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## ATTA ATTC ATTG ATTT CAAA CAAC CAAG CAAT CACA CACC CACG CACT CAGA CAGC CAGG
## 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## CAGT CATA CATC CATG CATT CCAA CCAC CCAG CCAT CCCA CCCC CCCG CCCT CCGA CCGC
## 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## CCGG CCGT CCTA CCTC CCTG CCTT CGAA CGAC CGAG CGAT CGCA CGCC CGCG CGCT CGGA
## 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## CGGC CGGG CGGT CGTA CGTC CGTG CGTT CTAA CTAC CTAG CTAT CTCA CTCC CTCG CTCT
## 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## CTGA CTGC CTGG CTGT CTTA CTTC CTTG CTTT GAAA GAAC GAAG GAAT GACA GACC GACG
## 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
## GACT GAGA GAGC GAGG GAGT GATA GATC GATG GATT GCAA GCAC GCAG GCAT GCCA GCCC
## 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## GCCG GCCT GCGA GCGC GCGG GCGT GCTA GCTC GCTG GCTT GGAA GGAC GGAG GGAT GGCA
## 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## GGCC GGCG GGCT GGGA GGGC GGGG GGGT GGTA GGTC GGTG GGTT GTAA GTAC GTAG GTAT
## 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
## GTCA GTCC GTCG GTCT GTGA GTGC GTGG GTGT GTTA GTTC GTTG GTTT TAAA TAAC TAAG
## 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## TAAT TACA TACC TACG TACT TAGA TAGC TAGG TAGT TATA TATC TATG TATT TCAA TCAC
## 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## TCAG TCAT TCCA TCCC TCCG TCCT TCGA TCGC TCGG TCGT TCTA TCTC TCTG TCTT TGAA
## 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## TGAC TGAG TGAT TGCA TGCC TGCG TGCT TGGA TGGC TGGG TGGT TGTA TGTC TGTG TGTT
## 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
## TTAA TTAC TTAG TTAT TTCA TTCC TTCG TTCT TTGA TTGC TTGG TTGT TTTA TTTC TTTG
## 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
## TTTT
## 0
# 计算相对于序列组的���特定位置���的碱基频率
nucleotideFrequencyAt(as(reads, "DNAStringSet"), 1)
## A C G T
## 1024 1024 1024 1024
dinucleotideFrequency(as(reads, "DNAStringSet"))
## AA AC AG AT CA CC CG CT GA GC GG GT TA TC TG TT
## [1,] 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [2,] 4 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
## [3,] 4 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
## [4,] 4 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
## [5,] 3 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0
## [6,] 3 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0
## [7,] 3 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0
## [8,] 3 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
## [9,] 3 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0
## [10,] 3 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0
## [11,] 3 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0
## [12,] 3 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0
## [13,] 3 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0
## [14,] 3 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0
## [15,] 3 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
## [16,] 3 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
## [17,] 3 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0
## [18,] 2 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0
## [19,] 2 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0
## [20,] 2 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0
## [21,] 2 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0
## [22,] 2 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0
## [23,] 2 1 0 0 0 1 1 0 0 0 0 0 0 0 0 0
## [24,] 2 1 0 0 0 1 0 1 0 0 0 0 0 0 0 0
## [25,] 2 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0
## [26,] 2 1 0 0 0 0 1 0 0 1 0 0 0 0 0 0
## [27,] 2 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0
## [28,] 2 1 0 0 0 0 1 0 0 0 0 1 0 0 0 0
## [29,] 2 1 0 0 0 0 0 1 0 0 0 0 1 0 0 0
## [30,] 2 1 0 0 0 0 0 1 0 0 0 0 0 1 0 0
## [31,] 2 1 0 0 0 0 0 1 0 0 0 0 0 0 1 0
## [32,] 2 1 0 0 0 0 0 1 0 0 0 0 0 0 0 1
## [33,] 3 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0
## [34,] 2 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0
## [35,] 2 0 2 0 0 0 0 0 1 0 0 0 0 0 0 0
## [36,] 2 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0
## [37,] 2 0 1 0 1 0 0 0 0 1 0 0 0 0 0 0
## [38,] 2 0 1 0 0 1 0 0 0 1 0 0 0 0 0 0
## [39,] 2 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0
## [40,] 2 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0
## [41,] 2 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0
## [42,] 2 0 1 0 0 0 0 0 0 1 1 0 0 0 0 0
## [43,] 2 0 1 0 0 0 0 0 0 0 2 0 0 0 0 0
## [44,] 2 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0
## [45,] 2 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0
## [46,] 2 0 1 0 0 0 0 0 0 0 0 1 0 1 0 0
## [47,] 2 0 1 0 0 0 0 0 0 0 0 1 0 0 1 0
## [48,] 2 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1
## [49,] 3 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0
## [50,] 2 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0
## [51,] 2 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0
## [52,] 2 0 0 2 0 0 0 0 0 0 0 0 1 0 0 0
## [53,] 2 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0
## [54,] 2 0 0 1 0 1 0 0 0 0 0 0 0 1 0 0
## [55,] 2 0 0 1 0 0 1 0 0 0 0 0 0 1 0 0
## [56,] 2 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0
## [57,] 2 0 0 1 0 0 0 0 1 0 0 0 0 0 1 0
## [58,] 2 0 0 1 0 0 0 0 0 1 0 0 0 0 1 0
## [59,] 2 0 0 1 0 0 0 0 0 0 1 0 0 0 1 0
## [60,] 2 0 0 1 0 0 0 0 0 0 0 1 0 0 1 0
## [61,] 2 0 0 1 0 0 0 0 0 0 0 0 1 0 0 1
## [62,] 2 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1
## [63,] 2 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1
## [64,] 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2
## [65,] 3 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0
## [66,] 2 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0
## [67,] 2 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0
## [68,] 2 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0
## [69,] 1 2 0 0 2 0 0 0 0 0 0 0 0 0 0 0
## [70,] 1 2 0 0 1 1 0 0 0 0 0 0 0 0 0 0
## [71,] 1 2 0 0 1 0 1 0 0 0 0 0 0 0 0 0
## [72,] 1 2 0 0 1 0 0 1 0 0 0 0 0 0 0 0
## [73,] 1 1 1 0 1 0 0 0 1 0 0 0 0 0 0 0
## [74,] 1 1 1 0 1 0 0 0 0 1 0 0 0 0 0 0
## [75,] 1 1 1 0 1 0 0 0 0 0 1 0 0 0 0 0
## [76,] 1 1 1 0 1 0 0 0 0 0 0 1 0 0 0 0
## [77,] 1 1 0 1 1 0 0 0 0 0 0 0 1 0 0 0
## [78,] 1 1 0 1 1 0 0 0 0 0 0 0 0 1 0 0
## [79,] 1 1 0 1 1 0 0 0 0 0 0 0 0 0 1 0
## [80,] 1 1 0 1 1 0 0 0 0 0 0 0 0 0 0 1
## [81,] 2 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0
## [82,] 1 2 0 0 1 1 0 0 0 0 0 0 0 0 0 0
## [83,] 1 1 1 0 1 1 0 0 0 0 0 0 0 0 0 0
## [84,] 1 1 0 1 1 1 0 0 0 0 0 0 0 0 0 0
## [85,] 1 1 0 0 1 2 0 0 0 0 0 0 0 0 0 0
## [86,] 1 1 0 0 0 3 0 0 0 0 0 0 0 0 0 0
## [87,] 1 1 0 0 0 2 1 0 0 0 0 0 0 0 0 0
## [88,] 1 1 0 0 0 2 0 1 0 0 0 0 0 0 0 0
## [89,] 1 1 0 0 0 1 1 0 1 0 0 0 0 0 0 0
## [90,] 1 1 0 0 0 1 1 0 0 1 0 0 0 0 0 0
## [91,] 1 1 0 0 0 1 1 0 0 0 1 0 0 0 0 0
## [92,] 1 1 0 0 0 1 1 0 0 0 0 1 0 0 0 0
## [93,] 1 1 0 0 0 1 0 1 0 0 0 0 1 0 0 0
## [94,] 1 1 0 0 0 1 0 1 0 0 0 0 0 1 0 0
## [95,] 1 1 0 0 0 1 0 1 0 0 0 0 0 0 1 0
## [96,] 1 1 0 0 0 1 0 1 0 0 0 0 0 0 0 1
## [97,] 2 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0
## [98,] 1 2 0 0 0 0 1 0 1 0 0 0 0 0 0 0
## [99,] 1 1 1 0 0 0 1 0 1 0 0 0 0 0 0 0
## [100,] 1 1 0 1 0 0 1 0 1 0 0 0 0 0 0 0
## [101,] 1 1 0 0 1 0 1 0 0 1 0 0 0 0 0 0
## [102,] 1 1 0 0 0 1 1 0 0 1 0 0 0 0 0 0
## [103,] 1 1 0 0 0 0 2 0 0 1 0 0 0 0 0 0
## [104,] 1 1 0 0 0 0 1 1 0 1 0 0 0 0 0 0
## [105,] 1 1 0 0 0 0 1 0 1 0 1 0 0 0 0 0
## [106,] 1 1 0 0 0 0 1 0 0 1 1 0 0 0 0 0
## [107,] 1 1 0 0 0 0 1 0 0 0 2 0 0 0 0 0
## [108,] 1 1 0 0 0 0 1 0 0 0 1 1 0 0 0 0
## [109,] 1 1 0 0 0 0 1 0 0 0 0 1 1 0 0 0
## [110,] 1 1 0 0 0 0 1 0 0 0 0 1 0 1 0 0
## [111,] 1 1 0 0 0 0 1 0 0 0 0 1 0 0 1 0
## [112,] 1 1 0 0 0 0 1 0 0 0 0 1 0 0 0 1
## [113,] 2 1 0 0 0 0 0 1 0 0 0 0 1 0 0 0
## [114,] 1 2 0 0 0 0 0 1 0 0 0 0 1 0 0 0
## [115,] 1 1 1 0 0 0 0 1 0 0 0 0 1 0 0 0
## [116,] 1 1 0 1 0 0 0 1 0 0 0 0 1 0 0 0
## [117,] 1 1 0 0 1 0 0 1 0 0 0 0 0 1 0 0
## [118,] 1 1 0 0 0 1 0 1 0 0 0 0 0 1 0 0
## [119,] 1 1 0 0 0 0 1 1 0 0 0 0 0 1 0 0
## [120,] 1 1 0 0 0 0 0 2 0 0 0 0 0 1 0 0
## [121,] 1 1 0 0 0 0 0 1 1 0 0 0 0 0 1 0
## [122,] 1 1 0 0 0 0 0 1 0 1 0 0 0 0 1 0
## [123,] 1 1 0 0 0 0 0 1 0 0 1 0 0 0 1 0
## [124,] 1 1 0 0 0 0 0 1 0 0 0 1 0 0 1 0
## [125,] 1 1 0 0 0 0 0 1 0 0 0 0 1 0 0 1
## [126,] 1 1 0 0 0 0 0 1 0 0 0 0 0 1 0 1
## [127,] 1 1 0 0 0 0 0 1 0 0 0 0 0 0 1 1
## [128,] 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 2
## [129,] 3 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0
## [130,] 2 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0
## [131,] 2 0 2 0 0 0 0 0 1 0 0 0 0 0 0 0
## [132,] 2 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0
## [133,] 1 1 1 0 1 0 0 0 1 0 0 0 0 0 0 0
## [134,] 1 1 1 0 0 1 0 0 1 0 0 0 0 0 0 0
## [135,] 1 1 1 0 0 0 1 0 1 0 0 0 0 0 0 0
## [136,] 1 1 1 0 0 0 0 1 1 0 0 0 0 0 0 0
## [137,] 1 0 2 0 0 0 0 0 2 0 0 0 0 0 0 0
## [138,] 1 0 2 0 0 0 0 0 1 1 0 0 0 0 0 0
## [139,] 1 0 2 0 0 0 0 0 1 0 1 0 0 0 0 0
## [140,] 1 0 2 0 0 0 0 0 1 0 0 1 0 0 0 0
## [141,] 1 0 1 1 0 0 0 0 1 0 0 0 1 0 0 0
## [142,] 1 0 1 1 0 0 0 0 1 0 0 0 0 1 0 0
## [143,] 1 0 1 1 0 0 0 0 1 0 0 0 0 0 1 0
## [144,] 1 0 1 1 0 0 0 0 1 0 0 0 0 0 0 1
## [145,] 2 0 1 0 1 0 0 0 0 1 0 0 0 0 0 0
## [146,] 1 1 1 0 1 0 0 0 0 1 0 0 0 0 0 0
## [147,] 1 0 2 0 1 0 0 0 0 1 0 0 0 0 0 0
## [148,] 1 0 1 1 1 0 0 0 0 1 0 0 0 0 0 0
## [149,] 1 0 1 0 1 1 0 0 0 1 0 0 0 0 0 0
## [150,] 1 0 1 0 0 2 0 0 0 1 0 0 0 0 0 0
## [151,] 1 0 1 0 0 1 1 0 0 1 0 0 0 0 0 0
## [152,] 1 0 1 0 0 1 0 1 0 1 0 0 0 0 0 0
## [153,] 1 0 1 0 0 0 1 0 1 1 0 0 0 0 0 0
## [154,] 1 0 1 0 0 0 1 0 0 2 0 0 0 0 0 0
## [155,] 1 0 1 0 0 0 1 0 0 1 1 0 0 0 0 0
## [156,] 1 0 1 0 0 0 1 0 0 1 0 1 0 0 0 0
## [157,] 1 0 1 0 0 0 0 1 0 1 0 0 1 0 0 0
## [158,] 1 0 1 0 0 0 0 1 0 1 0 0 0 1 0 0
## [159,] 1 0 1 0 0 0 0 1 0 1 0 0 0 0 1 0
## [160,] 1 0 1 0 0 0 0 1 0 1 0 0 0 0 0 1
## [161,] 2 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0
## [162,] 1 1 1 0 0 0 0 0 1 0 1 0 0 0 0 0
## [163,] 1 0 2 0 0 0 0 0 1 0 1 0 0 0 0 0
## [164,] 1 0 1 1 0 0 0 0 1 0 1 0 0 0 0 0
## [165,] 1 0 1 0 1 0 0 0 0 1 1 0 0 0 0 0
## [166,] 1 0 1 0 0 1 0 0 0 1 1 0 0 0 0 0
## [167,] 1 0 1 0 0 0 1 0 0 1 1 0 0 0 0 0
## [168,] 1 0 1 0 0 0 0 1 0 1 1 0 0 0 0 0
## [169,] 1 0 1 0 0 0 0 0 1 0 2 0 0 0 0 0
## [170,] 1 0 1 0 0 0 0 0 0 1 2 0 0 0 0 0
## [171,] 1 0 1 0 0 0 0 0 0 0 3 0 0 0 0 0
## [172,] 1 0 1 0 0 0 0 0 0 0 2 1 0 0 0 0
## [173,] 1 0 1 0 0 0 0 0 0 0 1 1 1 0 0 0
## [174,] 1 0 1 0 0 0 0 0 0 0 1 1 0 1 0 0
## [175,] 1 0 1 0 0 0 0 0 0 0 1 1 0 0 1 0
## [176,] 1 0 1 0 0 0 0 0 0 0 1 1 0 0 0 1
## [177,] 2 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0
## [178,] 1 1 1 0 0 0 0 0 0 0 0 1 1 0 0 0
## [179,] 1 0 2 0 0 0 0 0 0 0 0 1 1 0 0 0
## [180,] 1 0 1 1 0 0 0 0 0 0 0 1 1 0 0 0
## [181,] 1 0 1 0 1 0 0 0 0 0 0 1 0 1 0 0
## [182,] 1 0 1 0 0 1 0 0 0 0 0 1 0 1 0 0
## [183,] 1 0 1 0 0 0 1 0 0 0 0 1 0 1 0 0
## [184,] 1 0 1 0 0 0 0 1 0 0 0 1 0 1 0 0
## [185,] 1 0 1 0 0 0 0 0 1 0 0 1 0 0 1 0
## [186,] 1 0 1 0 0 0 0 0 0 1 0 1 0 0 1 0
## [187,] 1 0 1 0 0 0 0 0 0 0 1 1 0 0 1 0
## [188,] 1 0 1 0 0 0 0 0 0 0 0 2 0 0 1 0
## [189,] 1 0 1 0 0 0 0 0 0 0 0 1 1 0 0 1
## [190,] 1 0 1 0 0 0 0 0 0 0 0 1 0 1 0 1
## [191,] 1 0 1 0 0 0 0 0 0 0 0 1 0 0 1 1
## [192,] 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 2
## [193,] 3 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0
## [194,] 2 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0
## [195,] 2 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0
## [196,] 2 0 0 2 0 0 0 0 0 0 0 0 1 0 0 0
## [197,] 1 1 0 1 1 0 0 0 0 0 0 0 1 0 0 0
## [198,] 1 1 0 1 0 1 0 0 0 0 0 0 1 0 0 0
## [199,] 1 1 0 1 0 0 1 0 0 0 0 0 1 0 0 0
## [200,] 1 1 0 1 0 0 0 1 0 0 0 0 1 0 0 0
## [201,] 1 0 1 1 0 0 0 0 1 0 0 0 1 0 0 0
## [202,] 1 0 1 1 0 0 0 0 0 1 0 0 1 0 0 0
## [203,] 1 0 1 1 0 0 0 0 0 0 1 0 1 0 0 0
## [204,] 1 0 1 1 0 0 0 0 0 0 0 1 1 0 0 0
## [205,] 1 0 0 2 0 0 0 0 0 0 0 0 2 0 0 0
## [206,] 1 0 0 2 0 0 0 0 0 0 0 0 1 1 0 0
## [207,] 1 0 0 2 0 0 0 0 0 0 0 0 1 0 1 0
## [208,] 1 0 0 2 0 0 0 0 0 0 0 0 1 0 0 1
## [209,] 2 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0
## [210,] 1 1 0 1 1 0 0 0 0 0 0 0 0 1 0 0
## [211,] 1 0 1 1 1 0 0 0 0 0 0 0 0 1 0 0
## [212,] 1 0 0 2 1 0 0 0 0 0 0 0 0 1 0 0
## [213,] 1 0 0 1 1 1 0 0 0 0 0 0 0 1 0 0
## [214,] 1 0 0 1 0 2 0 0 0 0 0 0 0 1 0 0
## [215,] 1 0 0 1 0 1 1 0 0 0 0 0 0 1 0 0
## [216,] 1 0 0 1 0 1 0 1 0 0 0 0 0 1 0 0
## [217,] 1 0 0 1 0 0 1 0 1 0 0 0 0 1 0 0
## [218,] 1 0 0 1 0 0 1 0 0 1 0 0 0 1 0 0
## [219,] 1 0 0 1 0 0 1 0 0 0 1 0 0 1 0 0
## [220,] 1 0 0 1 0 0 1 0 0 0 0 1 0 1 0 0
## [221,] 1 0 0 1 0 0 0 1 0 0 0 0 1 1 0 0
## [222,] 1 0 0 1 0 0 0 1 0 0 0 0 0 2 0 0
## [223,] 1 0 0 1 0 0 0 1 0 0 0 0 0 1 1 0
## [224,] 1 0 0 1 0 0 0 1 0 0 0 0 0 1 0 1
## [225,] 2 0 0 1 0 0 0 0 1 0 0 0 0 0 1 0
## [226,] 1 1 0 1 0 0 0 0 1 0 0 0 0 0 1 0
## [227,] 1 0 1 1 0 0 0 0 1 0 0 0 0 0 1 0
## [228,] 1 0 0 2 0 0 0 0 1 0 0 0 0 0 1 0
## [229,] 1 0 0 1 1 0 0 0 0 1 0 0 0 0 1 0
## [230,] 1 0 0 1 0 1 0 0 0 1 0 0 0 0 1 0
## [231,] 1 0 0 1 0 0 1 0 0 1 0 0 0 0 1 0
## [232,] 1 0 0 1 0 0 0 1 0 1 0 0 0 0 1 0
## [233,] 1 0 0 1 0 0 0 0 1 0 1 0 0 0 1 0
## [234,] 1 0 0 1 0 0 0 0 0 1 1 0 0 0 1 0
## [235,] 1 0 0 1 0 0 0 0 0 0 2 0 0 0 1 0
## [236,] 1 0 0 1 0 0 0 0 0 0 1 1 0 0 1 0
## [237,] 1 0 0 1 0 0 0 0 0 0 0 1 1 0 1 0
## [238,] 1 0 0 1 0 0 0 0 0 0 0 1 0 1 1 0
## [239,] 1 0 0 1 0 0 0 0 0 0 0 1 0 0 2 0
## [240,] 1 0 0 1 0 0 0 0 0 0 0 1 0 0 1 1
## [241,] 2 0 0 1 0 0 0 0 0 0 0 0 1 0 0 1
## [242,] 1 1 0 1 0 0 0 0 0 0 0 0 1 0 0 1
## [243,] 1 0 1 1 0 0 0 0 0 0 0 0 1 0 0 1
## [244,] 1 0 0 2 0 0 0 0 0 0 0 0 1 0 0 1
## [245,] 1 0 0 1 1 0 0 0 0 0 0 0 0 1 0 1
## [246,] 1 0 0 1 0 1 0 0 0 0 0 0 0 1 0 1
## [247,] 1 0 0 1 0 0 1 0 0 0 0 0 0 1 0 1
## [248,] 1 0 0 1 0 0 0 1 0 0 0 0 0 1 0 1
## [249,] 1 0 0 1 0 0 0 0 1 0 0 0 0 0 1 1
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## [4012,] 0 0 0 0 0 0 0 0 0 0 2 1 0 0 1 1
## [4013,] 0 0 0 0 0 0 0 0 0 0 1 1 1 0 1 1
## [4014,] 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 1
## [4015,] 0 0 0 0 0 0 0 0 0 0 1 1 0 0 2 1
## [4016,] 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 2
## [4017,] 1 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1
## [4018,] 0 1 0 0 0 0 0 0 0 0 0 1 1 0 1 1
## [4019,] 0 0 1 0 0 0 0 0 0 0 0 1 1 0 1 1
## [4020,] 0 0 0 1 0 0 0 0 0 0 0 1 1 0 1 1
## [4021,] 0 0 0 0 1 0 0 0 0 0 0 1 0 1 1 1
## [4022,] 0 0 0 0 0 1 0 0 0 0 0 1 0 1 1 1
## [4023,] 0 0 0 0 0 0 1 0 0 0 0 1 0 1 1 1
## [4024,] 0 0 0 0 0 0 0 1 0 0 0 1 0 1 1 1
## [4025,] 0 0 0 0 0 0 0 0 1 0 0 1 0 0 2 1
## [4026,] 0 0 0 0 0 0 0 0 0 1 0 1 0 0 2 1
## [4027,] 0 0 0 0 0 0 0 0 0 0 1 1 0 0 2 1
## [4028,] 0 0 0 0 0 0 0 0 0 0 0 2 0 0 2 1
## [4029,] 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 2
## [4030,] 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 2
## [4031,] 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 2
## [4032,] 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 3
## [4033,] 2 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2
## [4034,] 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 2
## [4035,] 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 2
## [4036,] 1 0 0 1 0 0 0 0 0 0 0 0 1 0 0 2
## [4037,] 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 2
## [4038,] 0 1 0 0 0 1 0 0 0 0 0 0 1 0 0 2
## [4039,] 0 1 0 0 0 0 1 0 0 0 0 0 1 0 0 2
## [4040,] 0 1 0 0 0 0 0 1 0 0 0 0 1 0 0 2
## [4041,] 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 2
## [4042,] 0 0 1 0 0 0 0 0 0 1 0 0 1 0 0 2
## [4043,] 0 0 1 0 0 0 0 0 0 0 1 0 1 0 0 2
## [4044,] 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 2
## [4045,] 0 0 0 1 0 0 0 0 0 0 0 0 2 0 0 2
## [4046,] 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 2
## [4047,] 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 2
## [4048,] 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 3
## [4049,] 1 0 0 0 1 0 0 0 0 0 0 0 0 1 0 2
## [4050,] 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 2
## [4051,] 0 0 1 0 1 0 0 0 0 0 0 0 0 1 0 2
## [4052,] 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 2
## [4053,] 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 2
## [4054,] 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 2
## [4055,] 0 0 0 0 0 1 1 0 0 0 0 0 0 1 0 2
## [4056,] 0 0 0 0 0 1 0 1 0 0 0 0 0 1 0 2
## [4057,] 0 0 0 0 0 0 1 0 1 0 0 0 0 1 0 2
## [4058,] 0 0 0 0 0 0 1 0 0 1 0 0 0 1 0 2
## [4059,] 0 0 0 0 0 0 1 0 0 0 1 0 0 1 0 2
## [4060,] 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0 2
## [4061,] 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 2
## [4062,] 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0 2
## [4063,] 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 2
## [4064,] 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 3
## [4065,] 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1 2
## [4066,] 0 1 0 0 0 0 0 0 1 0 0 0 0 0 1 2
## [4067,] 0 0 1 0 0 0 0 0 1 0 0 0 0 0 1 2
## [4068,] 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1 2
## [4069,] 0 0 0 0 1 0 0 0 0 1 0 0 0 0 1 2
## [4070,] 0 0 0 0 0 1 0 0 0 1 0 0 0 0 1 2
## [4071,] 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1 2
## [4072,] 0 0 0 0 0 0 0 1 0 1 0 0 0 0 1 2
## [4073,] 0 0 0 0 0 0 0 0 1 0 1 0 0 0 1 2
## [4074,] 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 2
## [4075,] 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 2
## [4076,] 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 2
## [4077,] 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 2
## [4078,] 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 2
## [4079,] 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 2
## [4080,] 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 3
## [4081,] 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3
## [4082,] 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 3
## [4083,] 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 3
## [4084,] 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 3
## [4085,] 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 3
## [4086,] 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 3
## [4087,] 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 3
## [4088,] 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 3
## [4089,] 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 3
## [4090,] 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 3
## [4091,] 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 3
## [4092,] 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 3
## [4093,] 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 4
## [4094,] 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 4
## [4095,] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4
## [4096,] 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5
# A C T 加起来一共7个 G两个
x.freq <- letterFrequency(x, c("ACT", "G"))
is(x.freq)
## [1] "integer" "numeric" "vector"
## [4] "data.frameRowLabels" "integer_OR_Linteger" "atomic"
## [7] "vector_OR_factor"
x.freq
## A|C|T G
## 7 2
# 3 kmer slide window
x.freq <- letterFrequencyInSlidingView(x, 3, c("ACT", "G"), OR = 0)
is(x.freq)
## [1] "matrix" "array" "structure"
## [4] "vector" "vector_OR_factor"
x.freq
## A C T G
## [1,] 1 1 1 0
## [2,] 0 1 2 0
## [3,] 0 0 3 0
## [4,] 0 0 2 1
## [5,] 0 0 2 1
## [6,] 1 0 1 1
## [7,] 1 0 1 1
match
#countPattern
x <- DNAString("ACGTGTACCCGTAGT")
countPattern("ACGT", x)
## [1] 1
countPattern("ACGT", x, max.mismatch = 1)
## [1] 2
countPattern("NNGT", x, fixed = FALSE)
## [1] 4
vcountPattern("AAA", reads) %>% head()#计算多个碱基序列中的每一个
## [1] 4 3 3 3 2 2
#获得与模式匹配的位置
match <- matchPattern("NNGT", x, fixed = FALSE)
is(match)
## [1] "XStringViews" "Views" "List" "Vector"
## [5] "Annotated"
match
## Views on a 15-letter DNAString subject
## subject: ACGTGTACCCGTAGT
## views:
## start end width
## [1] 1 4 4 [ACGT]
## [2] 3 6 4 [GTGT]
## [3] 9 12 4 [CCGT]
## [4] 12 15 4 [TAGT]
#特定模式与匹配位置的进行比较时,您可以看到那些位置不匹配
mismatch("CCGT", match)
## [[1]]
## [1] 1
##
## [[2]]
## [1] 1 2
##
## [[3]]
## integer(0)
##
## [[4]]
## [1] 1 2
#获取多个碱基序列组中每一个的匹配位置
vmatch <- vmatchPattern("AAA", reads)
is(vmatch)
## [1] "ByPos_MIndex" "MIndex" "IRangesList" "RangesList"
## [5] "List" "Vector" "Annotated"
vmatch
## MIndex object of length 4096
## [[1]]
## IRanges object with 4 ranges and 0 metadata columns:
## start end width
## <integer> <integer> <integer>
## [1] 1 3 3
## [2] 2 4 3
## [3] 3 5 3
## [4] 4 6 3
##
## [[2]]
## IRanges object with 3 ranges and 0 metadata columns:
## start end width
## <integer> <integer> <integer>
## [1] 1 3 3
## [2] 2 4 3
## [3] 3 5 3
##
## [[3]]
## IRanges object with 3 ranges and 0 metadata columns:
## start end width
## <integer> <integer> <integer>
## [1] 1 3 3
## [2] 2 4 3
## [3] 3 5 3
##
## ...
## <4093 more elements>
vmatch[[2]]
## IRanges object with 3 ranges and 0 metadata columns:
## start end width
## <integer> <integer> <integer>
## [1] 1 3 3
## [2] 2 4 3
## [3] 3 5 3
#获得与相同长度的多个图案匹配的位置
v <- c("AAA", "GGG", "CCC")
dictionary <- DNAStringSet(v)
pdict <- PDict(dictionary)
is(pdict)
## [1] "TB_PDict" "PDict" "List" "Vector" "Annotated"
pdict
## TB_PDict object of length 3 and width 3 (preprocessing algo="ACtree2")
y <- DNAString("AAAGCGAGAAACTCGAGGGTCGATGGCCCC")
pdmatch <- matchPDict(pdict, y)
is(pdmatch)
## [1] "ByPos_MIndex" "MIndex" "IRangesList" "RangesList"
## [5] "List" "Vector" "Annotated"
pdmatch
## MIndex object of length 3
## [[1]]
## IRanges object with 2 ranges and 0 metadata columns:
## start end width
## <integer> <integer> <integer>
## [1] 1 3 3
## [2] 9 11 3
##
## [[2]]
## IRanges object with 1 range and 0 metadata columns:
## start end width
## <integer> <integer> <integer>
## [1] 17 19 3
##
## [[3]]
## IRanges object with 2 ranges and 0 metadata columns:
## start end width
## <integer> <integer> <integer>
## [1] 27 29 3
## [2] 28 30 3
#当左右图案匹配时将其删除,提取中间的序列,零宽断言
trimLRPatterns(Lpattern = "CAA", Rpattern = "CCC", y)
## 25-letter "DNAString" instance
## seq: AGCGAGAAACTCGAGGGTCGATGGC
#通过夹住左右图案来搜索匹配的部分序列,
matchLRPatterns(Lpattern = "AAA", Rpattern = "CCC", max.gaplength = 10,y)#max.gaplength: the max distance between the left and right parts of the pattern
## Views on a 30-letter DNAString subject
## subject: AAAGCGAGAAACTCGAGGGTCGATGGCCCC
## views: NONE
#用引物模拟PCR产物
matchProbePair(Fprobe = "GAG",
Rprobe = "GGG",
subject = y)#y: A DNAString object target sequence
## Views on a 30-letter DNAString subject
## subject: AAAGCGAGAAACTCGAGGGTCGATGGCCCC
## views:
## start end width
## [1] 6 14 9 [GAGAAACTC]
## [2] 17 29 13 [GGGTCGATGGCCC]
Palindromes 回文序列
y <- DNAString("AAAGCGAGAAACTCGAGGGTCGATGGCCCC")
p <- findPalindromes(y, min.armlength = 4, max.looplength = 10)
is(p)
## [1] "XStringViews" "Views" "List" "Vector"
## [5] "Annotated"
p
## Views on a 30-letter DNAString subject
## subject: AAAGCGAGAAACTCGAGGGTCGATGGCCCC
## views:
## start end width
## [1] 5 15 11 [CGAGAAACTCG]
## [2] 13 23 11 [TCGAGGGTCGA]
r <- y %>% complement %>% findPalindromes(., min.armlength = 4, max.looplength = 10)
is(r)
## [1] "XStringViews" "Views" "List" "Vector"
## [5] "Annotated"
r
## Views on a 30-letter DNAString subject
## subject: TTTCGCTCTTTGAGCTCCCAGCTACCGGGG
## views:
## start end width
## [1] 5 15 11 [GCTCTTTGAGC]
## [2] 13 23 11 [AGCTCCCAGCT]
alignment
#全局比对
pairwiseAlignment(x, y, type = "global")
## Global PairwiseAlignmentsSingleSubject (1 of 1)
## pattern: [1] A---CGTGTACCCGTAGT
## subject: [1] AAAGCGAGAAACTCGAGG
## score: -105.4409
#局部比对
pairwiseAlignment(x, y, type = "local")
## Local PairwiseAlignmentsSingleSubject (1 of 1)
## pattern: [8] CCC
## subject: [27] CCC
## score: 5.945268
fasta
fasta <- readDNAStringSet("/Users/zero/myrepo/jixingBlogdown/data/sequence_numb_human_primer_inter_range.fasta", format = "fasta")
is(fasta)
## [1] "DNAStringSet" "XStringSet" "XRawList" "XVectorList"
## [5] "List" "Vector" "Annotated"
fasta
## A DNAStringSet instance of length 1
## width seq names
## [1] 227 GCAACCTAGTGTAAAGTCCAC...ATCCCTAACTCAGGAAGCTA NC_000014.9:c7346...
names(fasta)
## [1] "NC_000014.9:c73462350-73462124 Homo sapiens chromosome 14, GRCh38.p12 Primary Assembly"
length(fasta)
## [1] 1
width(fasta)
## [1] 227
fasta
## A DNAStringSet instance of length 1
## width seq names
## [1] 227 GCAACCTAGTGTAAAGTCCAC...ATCCCTAACTCAGGAAGCTA NC_000014.9:c7346...
fastq <- readDNAStringSet("/Users/zero/myrepo/jixingBlogdown/data/test.fastq", format = "fastq")
fastq
## A DNAStringSet instance of length 1
## width seq names
## [1] 227 GCAACCTAGTGTAAAGTCCAC...ATCCCTAACTCAGGAAGCTA NC_000014.9:c7346...
#fa2fq
writeXStringSet(fasta, file="test.fastq", format = "fastq")
#fq2fa
writeXStringSet(fastq, file = "test.fa", format = "fasta")
BSgenome
library(BSgenome)
ls("package:BSgenome")
## [1] "alphabetFrequency"
## [2] "as.data.frame"
## [3] "as.data.frame.BSgenomeViews"
## [4] "as.list"
## [5] "available.genomes"
## [6] "available.SNPs"
## [7] "batchsizes"
## [8] "blocksizes"
## [9] "breakpoints"
## [10] "bsapply"
## [11] "BSgenome"
## [12] "BSgenomeViews"
## [13] "coerce"
## [14] "commonName"
## [15] "compatibleGenomes"
## [16] "consensusMatrix"
## [17] "consensusString"
## [18] "countPWM"
## [19] "elementNROWS"
## [20] "end"
## [21] "export"
## [22] "forgeBSgenomeDataPkg"
## [23] "forgeMaskedBSgenomeDataPkg"
## [24] "forgeMasksFiles"
## [25] "forgeSeqFiles"
## [26] "forgeSeqlengthsFile"
## [27] "getBatchesByOverlapsFromOnDiskLongTable"
## [28] "getBatchesBySeqnameFromOnDiskLongTable"
## [29] "getBatchesFromOnDiskLongTable"
## [30] "getBatchesFromOnDiskLongTable_old"
## [31] "getBSgenome"
## [32] "getRowsByIdFromOnDiskLongTable"
## [33] "getRowsByIdFromOnDiskLongTable_old"
## [34] "getRowsByIndexFromOnDiskLongTable_old"
## [35] "getRowsFromOnDiskLongTable"
## [36] "getSeq"
## [37] "granges"
## [38] "hasOnlyBaseLetters"
## [39] "injectSNPs"
## [40] "installed.genomes"
## [41] "installed.SNPs"
## [42] "letterFrequency"
## [43] "MaskedBSgenome"
## [44] "masknames"
## [45] "matchPWM"
## [46] "mseqnames"
## [47] "nchar"
## [48] "newSNPlocs"
## [49] "newXtraSNPlocs"
## [50] "nucleotideFrequencyAt"
## [51] "oligonucleotideFrequency"
## [52] "OnDiskLongTable"
## [53] "OnDiskLongTable_old"
## [54] "organism"
## [55] "provider"
## [56] "providerVersion"
## [57] "ranges"
## [58] "referenceGenome"
## [59] "releaseDate"
## [60] "releaseName"
## [61] "rowids"
## [62] "saveAsOnDiskLongTable_old"
## [63] "saveRowidsForOnDiskLongTable_old"
## [64] "score"
## [65] "seqinfo"
## [66] "seqinfo<-"
## [67] "seqnames"
## [68] "seqnames<-"
## [69] "seqtype"
## [70] "show"
## [71] "snpcount"
## [72] "snpid2alleles"
## [73] "snpid2grange"
## [74] "snpid2loc"
## [75] "snplocs"
## [76] "SNPlocs_pkgname"
## [77] "snpsById"
## [78] "snpsByOverlaps"
## [79] "snpsBySeqname"
## [80] "sourceUrl"
## [81] "spatialIndex"
## [82] "start"
## [83] "strand"
## [84] "subject"
## [85] "uniqueLetters"
## [86] "vcountPattern"
## [87] "Views"
## [88] "vmatchPattern"
## [89] "width"
## [90] "writeBSgenomeToFasta"
## [91] "writeBSgenomeToTwobit"
## [92] "writeOnDiskLongTable"
## [93] "writeOnDiskLongTableRowids"
available.genomes()
## [1] "BSgenome.Alyrata.JGI.v1"
## [2] "BSgenome.Amellifera.BeeBase.assembly4"
## [3] "BSgenome.Amellifera.UCSC.apiMel2"
## [4] "BSgenome.Amellifera.UCSC.apiMel2.masked"
## [5] "BSgenome.Athaliana.TAIR.04232008"
## [6] "BSgenome.Athaliana.TAIR.TAIR9"
## [7] "BSgenome.Btaurus.UCSC.bosTau3"
## [8] "BSgenome.Btaurus.UCSC.bosTau3.masked"
## [9] "BSgenome.Btaurus.UCSC.bosTau4"
## [10] "BSgenome.Btaurus.UCSC.bosTau4.masked"
## [11] "BSgenome.Btaurus.UCSC.bosTau6"
## [12] "BSgenome.Btaurus.UCSC.bosTau6.masked"
## [13] "BSgenome.Btaurus.UCSC.bosTau8"
## [14] "BSgenome.Celegans.UCSC.ce10"
## [15] "BSgenome.Celegans.UCSC.ce11"
## [16] "BSgenome.Celegans.UCSC.ce2"
## [17] "BSgenome.Celegans.UCSC.ce6"
## [18] "BSgenome.Cfamiliaris.UCSC.canFam2"
## [19] "BSgenome.Cfamiliaris.UCSC.canFam2.masked"
## [20] "BSgenome.Cfamiliaris.UCSC.canFam3"
## [21] "BSgenome.Cfamiliaris.UCSC.canFam3.masked"
## [22] "BSgenome.Dmelanogaster.UCSC.dm2"
## [23] "BSgenome.Dmelanogaster.UCSC.dm2.masked"
## [24] "BSgenome.Dmelanogaster.UCSC.dm3"
## [25] "BSgenome.Dmelanogaster.UCSC.dm3.masked"
## [26] "BSgenome.Dmelanogaster.UCSC.dm6"
## [27] "BSgenome.Drerio.UCSC.danRer10"
## [28] "BSgenome.Drerio.UCSC.danRer5"
## [29] "BSgenome.Drerio.UCSC.danRer5.masked"
## [30] "BSgenome.Drerio.UCSC.danRer6"
## [31] "BSgenome.Drerio.UCSC.danRer6.masked"
## [32] "BSgenome.Drerio.UCSC.danRer7"
## [33] "BSgenome.Drerio.UCSC.danRer7.masked"
## [34] "BSgenome.Ecoli.NCBI.20080805"
## [35] "BSgenome.Gaculeatus.UCSC.gasAcu1"
## [36] "BSgenome.Gaculeatus.UCSC.gasAcu1.masked"
## [37] "BSgenome.Ggallus.UCSC.galGal3"
## [38] "BSgenome.Ggallus.UCSC.galGal3.masked"
## [39] "BSgenome.Ggallus.UCSC.galGal4"
## [40] "BSgenome.Ggallus.UCSC.galGal4.masked"
## [41] "BSgenome.Ggallus.UCSC.galGal5"
## [42] "BSgenome.Hsapiens.1000genomes.hs37d5"
## [43] "BSgenome.Hsapiens.NCBI.GRCh38"
## [44] "BSgenome.Hsapiens.UCSC.hg17"
## [45] "BSgenome.Hsapiens.UCSC.hg17.masked"
## [46] "BSgenome.Hsapiens.UCSC.hg18"
## [47] "BSgenome.Hsapiens.UCSC.hg18.masked"
## [48] "BSgenome.Hsapiens.UCSC.hg19"
## [49] "BSgenome.Hsapiens.UCSC.hg19.masked"
## [50] "BSgenome.Hsapiens.UCSC.hg38"
## [51] "BSgenome.Hsapiens.UCSC.hg38.masked"
## [52] "BSgenome.Mfascicularis.NCBI.5.0"
## [53] "BSgenome.Mfuro.UCSC.musFur1"
## [54] "BSgenome.Mmulatta.UCSC.rheMac2"
## [55] "BSgenome.Mmulatta.UCSC.rheMac2.masked"
## [56] "BSgenome.Mmulatta.UCSC.rheMac3"
## [57] "BSgenome.Mmulatta.UCSC.rheMac3.masked"
## [58] "BSgenome.Mmulatta.UCSC.rheMac8"
## [59] "BSgenome.Mmusculus.UCSC.mm10"
## [60] "BSgenome.Mmusculus.UCSC.mm10.masked"
## [61] "BSgenome.Mmusculus.UCSC.mm8"
## [62] "BSgenome.Mmusculus.UCSC.mm8.masked"
## [63] "BSgenome.Mmusculus.UCSC.mm9"
## [64] "BSgenome.Mmusculus.UCSC.mm9.masked"
## [65] "BSgenome.Osativa.MSU.MSU7"
## [66] "BSgenome.Ptroglodytes.UCSC.panTro2"
## [67] "BSgenome.Ptroglodytes.UCSC.panTro2.masked"
## [68] "BSgenome.Ptroglodytes.UCSC.panTro3"
## [69] "BSgenome.Ptroglodytes.UCSC.panTro3.masked"
## [70] "BSgenome.Ptroglodytes.UCSC.panTro5"
## [71] "BSgenome.Rnorvegicus.UCSC.rn4"
## [72] "BSgenome.Rnorvegicus.UCSC.rn4.masked"
## [73] "BSgenome.Rnorvegicus.UCSC.rn5"
## [74] "BSgenome.Rnorvegicus.UCSC.rn5.masked"
## [75] "BSgenome.Rnorvegicus.UCSC.rn6"
## [76] "BSgenome.Scerevisiae.UCSC.sacCer1"
## [77] "BSgenome.Scerevisiae.UCSC.sacCer2"
## [78] "BSgenome.Scerevisiae.UCSC.sacCer3"
## [79] "BSgenome.Sscrofa.UCSC.susScr3"
## [80] "BSgenome.Sscrofa.UCSC.susScr3.masked"
## [81] "BSgenome.Tgondii.ToxoDB.7.0"
## [82] "BSgenome.Tguttata.UCSC.taeGut1"
## [83] "BSgenome.Tguttata.UCSC.taeGut1.masked"
## [84] "BSgenome.Tguttata.UCSC.taeGut2"
## [85] "BSgenome.Vvinifera.URGI.IGGP12Xv0"
## [86] "BSgenome.Vvinifera.URGI.IGGP12Xv2"
## [87] "BSgenome.Vvinifera.URGI.IGGP8X"
installed.genomes()
## [1] "BSgenome.Hsapiens.UCSC.hg19" "BSgenome.Mmusculus.UCSC.mm10"
## [3] "BSgenome.Rnorvegicus.UCSC.rn5"
library("BSgenome.Mmusculus.UCSC.mm10")
ls("package:BSgenome.Mmusculus.UCSC.mm10")
## [1] "BSgenome.Mmusculus.UCSC.mm10" "Mmusculus"
Mmusculus
## Mouse genome:
## # organism: Mus musculus (Mouse)
## # provider: UCSC
## # provider version: mm10
## # release date: Dec. 2011
## # release name: Genome Reference Consortium GRCm38
## # 66 sequences:
## # chr1 chr2 chr3
## # chr4 chr5 chr6
## # chr7 chr8 chr9
## # chr10 chr11 chr12
## # chr13 chr14 chr15
## # ... ... ...
## # chrUn_GL456372 chrUn_GL456378 chrUn_GL456379
## # chrUn_GL456381 chrUn_GL456382 chrUn_GL456383
## # chrUn_GL456385 chrUn_GL456387 chrUn_GL456389
## # chrUn_GL456390 chrUn_GL456392 chrUn_GL456393
## # chrUn_GL456394 chrUn_GL456396 chrUn_JH584304
## # (use 'seqnames()' to see all the sequence names, use the '$' or '[['
## # operator to access a given sequence)
is(Mmusculus)
## [1] "BSgenome" "GenomeDescription"
seqinfo(Mmusculus)
## Seqinfo object with 66 sequences (1 circular) from mm10 genome:
## seqnames seqlengths isCircular genome
## chr1 195471971 FALSE mm10
## chr2 182113224 FALSE mm10
## chr3 160039680 FALSE mm10
## chr4 156508116 FALSE mm10
## chr5 151834684 FALSE mm10
## ... ... ... ...
## chrUn_GL456392 23629 FALSE mm10
## chrUn_GL456393 55711 FALSE mm10
## chrUn_GL456394 24323 FALSE mm10
## chrUn_GL456396 21240 FALSE mm10
## chrUn_JH584304 114452 FALSE mm10
seqnames(Mmusculus)
## [1] "chr1" "chr2" "chr3"
## [4] "chr4" "chr5" "chr6"
## [7] "chr7" "chr8" "chr9"
## [10] "chr10" "chr11" "chr12"
## [13] "chr13" "chr14" "chr15"
## [16] "chr16" "chr17" "chr18"
## [19] "chr19" "chrX" "chrY"
## [22] "chrM" "chr1_GL456210_random" "chr1_GL456211_random"
## [25] "chr1_GL456212_random" "chr1_GL456213_random" "chr1_GL456221_random"
## [28] "chr4_GL456216_random" "chr4_GL456350_random" "chr4_JH584292_random"
## [31] "chr4_JH584293_random" "chr4_JH584294_random" "chr4_JH584295_random"
## [34] "chr5_GL456354_random" "chr5_JH584296_random" "chr5_JH584297_random"
## [37] "chr5_JH584298_random" "chr5_JH584299_random" "chr7_GL456219_random"
## [40] "chrX_GL456233_random" "chrY_JH584300_random" "chrY_JH584301_random"
## [43] "chrY_JH584302_random" "chrY_JH584303_random" "chrUn_GL456239"
## [46] "chrUn_GL456359" "chrUn_GL456360" "chrUn_GL456366"
## [49] "chrUn_GL456367" "chrUn_GL456368" "chrUn_GL456370"
## [52] "chrUn_GL456372" "chrUn_GL456378" "chrUn_GL456379"
## [55] "chrUn_GL456381" "chrUn_GL456382" "chrUn_GL456383"
## [58] "chrUn_GL456385" "chrUn_GL456387" "chrUn_GL456389"
## [61] "chrUn_GL456390" "chrUn_GL456392" "chrUn_GL456393"
## [64] "chrUn_GL456394" "chrUn_GL456396" "chrUn_JH584304"
seqlevels(Mmusculus)
## [1] "chr1" "chr2" "chr3"
## [4] "chr4" "chr5" "chr6"
## [7] "chr7" "chr8" "chr9"
## [10] "chr10" "chr11" "chr12"
## [13] "chr13" "chr14" "chr15"
## [16] "chr16" "chr17" "chr18"
## [19] "chr19" "chrX" "chrY"
## [22] "chrM" "chr1_GL456210_random" "chr1_GL456211_random"
## [25] "chr1_GL456212_random" "chr1_GL456213_random" "chr1_GL456221_random"
## [28] "chr4_GL456216_random" "chr4_GL456350_random" "chr4_JH584292_random"
## [31] "chr4_JH584293_random" "chr4_JH584294_random" "chr4_JH584295_random"
## [34] "chr5_GL456354_random" "chr5_JH584296_random" "chr5_JH584297_random"
## [37] "chr5_JH584298_random" "chr5_JH584299_random" "chr7_GL456219_random"
## [40] "chrX_GL456233_random" "chrY_JH584300_random" "chrY_JH584301_random"
## [43] "chrY_JH584302_random" "chrY_JH584303_random" "chrUn_GL456239"
## [46] "chrUn_GL456359" "chrUn_GL456360" "chrUn_GL456366"
## [49] "chrUn_GL456367" "chrUn_GL456368" "chrUn_GL456370"
## [52] "chrUn_GL456372" "chrUn_GL456378" "chrUn_GL456379"
## [55] "chrUn_GL456381" "chrUn_GL456382" "chrUn_GL456383"
## [58] "chrUn_GL456385" "chrUn_GL456387" "chrUn_GL456389"
## [61] "chrUn_GL456390" "chrUn_GL456392" "chrUn_GL456393"
## [64] "chrUn_GL456394" "chrUn_GL456396" "chrUn_JH584304"
seqlengths(Mmusculus)
## chr1 chr2 chr3
## 195471971 182113224 160039680
## chr4 chr5 chr6
## 156508116 151834684 149736546
## chr7 chr8 chr9
## 145441459 129401213 124595110
## chr10 chr11 chr12
## 130694993 122082543 120129022
## chr13 chr14 chr15
## 120421639 124902244 104043685
## chr16 chr17 chr18
## 98207768 94987271 90702639
## chr19 chrX chrY
## 61431566 171031299 91744698
## chrM chr1_GL456210_random chr1_GL456211_random
## 16299 169725 241735
## chr1_GL456212_random chr1_GL456213_random chr1_GL456221_random
## 153618 39340 206961
## chr4_GL456216_random chr4_GL456350_random chr4_JH584292_random
## 66673 227966 14945
## chr4_JH584293_random chr4_JH584294_random chr4_JH584295_random
## 207968 191905 1976
## chr5_GL456354_random chr5_JH584296_random chr5_JH584297_random
## 195993 199368 205776
## chr5_JH584298_random chr5_JH584299_random chr7_GL456219_random
## 184189 953012 175968
## chrX_GL456233_random chrY_JH584300_random chrY_JH584301_random
## 336933 182347 259875
## chrY_JH584302_random chrY_JH584303_random chrUn_GL456239
## 155838 158099 40056
## chrUn_GL456359 chrUn_GL456360 chrUn_GL456366
## 22974 31704 47073
## chrUn_GL456367 chrUn_GL456368 chrUn_GL456370
## 42057 20208 26764
## chrUn_GL456372 chrUn_GL456378 chrUn_GL456379
## 28664 31602 72385
## chrUn_GL456381 chrUn_GL456382 chrUn_GL456383
## 25871 23158 38659
## chrUn_GL456385 chrUn_GL456387 chrUn_GL456389
## 35240 24685 28772
## chrUn_GL456390 chrUn_GL456392 chrUn_GL456393
## 24668 23629 55711
## chrUn_GL456394 chrUn_GL456396 chrUn_JH584304
## 24323 21240 114452
isCircular(Mmusculus)
## chr1 chr2 chr3
## FALSE FALSE FALSE
## chr4 chr5 chr6
## FALSE FALSE FALSE
## chr7 chr8 chr9
## FALSE FALSE FALSE
## chr10 chr11 chr12
## FALSE FALSE FALSE
## chr13 chr14 chr15
## FALSE FALSE FALSE
## chr16 chr17 chr18
## FALSE FALSE FALSE
## chr19 chrX chrY
## FALSE FALSE FALSE
## chrM chr1_GL456210_random chr1_GL456211_random
## TRUE FALSE FALSE
## chr1_GL456212_random chr1_GL456213_random chr1_GL456221_random
## FALSE FALSE FALSE
## chr4_GL456216_random chr4_GL456350_random chr4_JH584292_random
## FALSE FALSE FALSE
## chr4_JH584293_random chr4_JH584294_random chr4_JH584295_random
## FALSE FALSE FALSE
## chr5_GL456354_random chr5_JH584296_random chr5_JH584297_random
## FALSE FALSE FALSE
## chr5_JH584298_random chr5_JH584299_random chr7_GL456219_random
## FALSE FALSE FALSE
## chrX_GL456233_random chrY_JH584300_random chrY_JH584301_random
## FALSE FALSE FALSE
## chrY_JH584302_random chrY_JH584303_random chrUn_GL456239
## FALSE FALSE FALSE
## chrUn_GL456359 chrUn_GL456360 chrUn_GL456366
## FALSE FALSE FALSE
## chrUn_GL456367 chrUn_GL456368 chrUn_GL456370
## FALSE FALSE FALSE
## chrUn_GL456372 chrUn_GL456378 chrUn_GL456379
## FALSE FALSE FALSE
## chrUn_GL456381 chrUn_GL456382 chrUn_GL456383
## FALSE FALSE FALSE
## chrUn_GL456385 chrUn_GL456387 chrUn_GL456389
## FALSE FALSE FALSE
## chrUn_GL456390 chrUn_GL456392 chrUn_GL456393
## FALSE FALSE FALSE
## chrUn_GL456394 chrUn_GL456396 chrUn_JH584304
## FALSE FALSE FALSE
mseqnames(Mmusculus)
## NULL
genome(Mmusculus)
## chr1 chr2 chr3
## "mm10" "mm10" "mm10"
## chr4 chr5 chr6
## "mm10" "mm10" "mm10"
## chr7 chr8 chr9
## "mm10" "mm10" "mm10"
## chr10 chr11 chr12
## "mm10" "mm10" "mm10"
## chr13 chr14 chr15
## "mm10" "mm10" "mm10"
## chr16 chr17 chr18
## "mm10" "mm10" "mm10"
## chr19 chrX chrY
## "mm10" "mm10" "mm10"
## chrM chr1_GL456210_random chr1_GL456211_random
## "mm10" "mm10" "mm10"
## chr1_GL456212_random chr1_GL456213_random chr1_GL456221_random
## "mm10" "mm10" "mm10"
## chr4_GL456216_random chr4_GL456350_random chr4_JH584292_random
## "mm10" "mm10" "mm10"
## chr4_JH584293_random chr4_JH584294_random chr4_JH584295_random
## "mm10" "mm10" "mm10"
## chr5_GL456354_random chr5_JH584296_random chr5_JH584297_random
## "mm10" "mm10" "mm10"
## chr5_JH584298_random chr5_JH584299_random chr7_GL456219_random
## "mm10" "mm10" "mm10"
## chrX_GL456233_random chrY_JH584300_random chrY_JH584301_random
## "mm10" "mm10" "mm10"
## chrY_JH584302_random chrY_JH584303_random chrUn_GL456239
## "mm10" "mm10" "mm10"
## chrUn_GL456359 chrUn_GL456360 chrUn_GL456366
## "mm10" "mm10" "mm10"
## chrUn_GL456367 chrUn_GL456368 chrUn_GL456370
## "mm10" "mm10" "mm10"
## chrUn_GL456372 chrUn_GL456378 chrUn_GL456379
## "mm10" "mm10" "mm10"
## chrUn_GL456381 chrUn_GL456382 chrUn_GL456383
## "mm10" "mm10" "mm10"
## chrUn_GL456385 chrUn_GL456387 chrUn_GL456389
## "mm10" "mm10" "mm10"
## chrUn_GL456390 chrUn_GL456392 chrUn_GL456393
## "mm10" "mm10" "mm10"
## chrUn_GL456394 chrUn_GL456396 chrUn_JH584304
## "mm10" "mm10" "mm10"
Mmusculus[["chr19"]]
## 61431566-letter "DNAString" instance
## seq: NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN...NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
Mmusculus[[seqnames(Mmusculus)[19]]]
## 61431566-letter "DNAString" instance
## seq: NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN...NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
Mmusculus$chr19
## 61431566-letter "DNAString" instance
## seq: NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN...NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
mm9.chr19 <- BSgenome.Mmusculus.UCSC.mm10::Mmusculus$chr19
is(mm9.chr19)
## [1] "DNAString" "XString" "XRaw" "XVector" "Vector" "Annotated"
mm9.chr19
## 61431566-letter "DNAString" instance
## seq: NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN...NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
countPattern("CACGTG", mm9.chr19)
## [1] 6849
masknames(Mmusculus)
## NULL
available.SNPs()
## [1] "SNPlocs.Hsapiens.dbSNP.20101109"
## [2] "SNPlocs.Hsapiens.dbSNP.20120608"
## [3] "SNPlocs.Hsapiens.dbSNP141.GRCh38"
## [4] "SNPlocs.Hsapiens.dbSNP142.GRCh37"
## [5] "SNPlocs.Hsapiens.dbSNP144.GRCh37"
## [6] "SNPlocs.Hsapiens.dbSNP144.GRCh38"
## [7] "SNPlocs.Hsapiens.dbSNP149.GRCh38"
## [8] "SNPlocs.Hsapiens.dbSNP150.GRCh38"
## [9] "XtraSNPlocs.Hsapiens.dbSNP141.GRCh38"
## [10] "XtraSNPlocs.Hsapiens.dbSNP144.GRCh37"
## [11] "XtraSNPlocs.Hsapiens.dbSNP144.GRCh38"